Journal: Bioengineered

Article Title: Long non-coding RNA LINC00997 silencing inhibits the progression and metastasis of colorectal cancer by sponging miR-512-3p.

doi: 10.1080/21655979.2021.1882164

Figure Lengend Snippet: Figure 3. LINC00997 silencing suppressed the migration and invasion of CRC cells. (a) LINC00997 level in several CRC cell lines was detected using RT-qPCR. **P < 0.01, ***P < 0.001 vs. HIEC. (b) The level of LINC00997 was tested with RT-qPCR after transfection with shRNA-LINC00997 into HCT116 cells. **P < 0.01, ***P < 0.001. (c and d) Cell migration was tested using wound healing assay. (e and f) The invasive ability of HCT116 cells was determined with Transwell assay. (g) Western blot analysis was used to examine the expression of MMP2 and MMP7. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Several human CRC cell lines (SW480, SW1417, SW620, Caco2, LS174T, HCT15, LoVo, HT29 and HCT116) and the normal intestinal epithelial cell line (HIEC) used in this study were obtained from American Type Culture Collection (Manassas, VA, USA).

Techniques: Migration, Quantitative RT-PCR, Transfection, shRNA, Wound Healing Assay, Transwell Assay, Western Blot, Expressing

Journal: American Journal of Cancer Research

Article Title: Activation of the IL-17 signalling pathway by the CXCL17-GPR35 axis affects drug resistance and colorectal cancer tumorigenesis

doi:

Figure Lengend Snippet: Chemokine CXCL17 and its receptor GPR35 exhibit higher levels in CRC drug-resistant tissues and cells. A. Differential gene volcano plot between OXA-sensitive and OXA-resistant groups in CRC tissues; B. Box plot of CXCL17 expression in OXA-sensitive and OXA-resistant tumor tissues; C. Western-blot detection of CXCL17 and GPR35 protein expression levels in FHC and HCT15 cells; D. RT-qPCR detection of mRNA expression of CXCL17 and GPR35. **P < 0.01, compared with the FHC group.

Article Snippet: Drug-resistant human colorectal cancer cells (HCT15) were cultured in RPMI 1640 medium supplemented with 10% foetal bovine serum, 1% penicillin and streptomycin, and 500 ng/ml taxol (a microtubule-stabilising drug used to treat cancer) (Med Chem Express, Monmouth Junction, NJ, USA).

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: American Journal of Cancer Research

Article Title: Activation of the IL-17 signalling pathway by the CXCL17-GPR35 axis affects drug resistance and colorectal cancer tumorigenesis

doi:

Figure Lengend Snippet: CXCL17 plays a critical role in proliferation and drug-resistance of HCT15 cells. A, B. CCK-8 assay was performed to detect cell viability after the treatment with CXCL17 silence or different concentrations of taxol; C. Wound-healing assay for cell migration ability of HCT15 cells upon siNC or si-CXCL17 transfection; D. Transwell assay was applied to analyze cell invasion ability; E. Cell cycle was detected by flow cytometry; F. Cell apoptosis was detected by flow cytometry. Scale bar was 50 μm. Compared with siNC group, *P < 0.05; **P < 0.01.

Article Snippet: Drug-resistant human colorectal cancer cells (HCT15) were cultured in RPMI 1640 medium supplemented with 10% foetal bovine serum, 1% penicillin and streptomycin, and 500 ng/ml taxol (a microtubule-stabilising drug used to treat cancer) (Med Chem Express, Monmouth Junction, NJ, USA).

Techniques: CCK-8 Assay, Wound Healing Assay, Migration, Transfection, Transwell Assay, Flow Cytometry

Journal: American Journal of Cancer Research

Article Title: Activation of the IL-17 signalling pathway by the CXCL17-GPR35 axis affects drug resistance and colorectal cancer tumorigenesis

doi:

Figure Lengend Snippet: IL-17 signaling pathway is involved in tumorigenesis and drug resistance of HCT15 cells. A. CCK-8 assay was performed to detect cell viability of HCT15 cells with or without CXCL17 silence upon IL-17A supplementation; B. CCK-8 assay for cell resistance was applied to detect the cell viability of HCT15 cells with or without CXCL17 silence which treated with different concentrations of taxol; C. Wound-healing assay was used to evaluate cell migration ability of HCT15 cells treated with IL-17A followed by siNC or si-CXCL17 transfection; D. Transwell assay was applied to analyze cell invasion ability of HCT15 treated with IL-17A followed by siNC or si-CXCL17 transfection; E. Flow cytometry was used to detect the cell cycle of HCT15 cells treated with IL-17A followed by siNC or si-CXCL17 transfection; F. Flow cytometry was used to detect the cell apoptosis of HCT15 cells treated with IL-17A followed by siNC or si-CXCL17 transfection. Scale bar was 50 μm. Compared with the siNC group, **P < 0.01; compared with the si-CXCL17 group, #P < 0.05; ##P < 0.01.

Article Snippet: Drug-resistant human colorectal cancer cells (HCT15) were cultured in RPMI 1640 medium supplemented with 10% foetal bovine serum, 1% penicillin and streptomycin, and 500 ng/ml taxol (a microtubule-stabilising drug used to treat cancer) (Med Chem Express, Monmouth Junction, NJ, USA).

Techniques: CCK-8 Assay, Wound Healing Assay, Migration, Transfection, Transwell Assay, Flow Cytometry